Antimicrobial Activity of Solvent Extracts of Ocimum sanctum, Azadirachta Indica and Phyllanthus amarus Against Clinical Pathogens

In the present study, leaves of Ocimum sanctum, Azadirachta indica and Phyllanthus amarus were collected and the collected leaves were shade dried and powdered by hand crushing. The preparations of different leaves extract was done through modified method. Three different solvents viz., methanol, chloroform and ethyl acetate were used to study the antimicrobial activity of herbal plants. Disc diffusion method was adopted for evaluation of antimicrobial activity of five different medicinal leaves. The antimicrobial activity of methanol, chloroform and ethyl acetate leaf extract of Ocimum sanctum, Azadirachta indica and Phyllanthus amarus were studied in different concentrations (100 mg/ml, 200 mg/ml, 300 mg/ml). Among the three concentrations (100 mg/ml, 200, 300 mg/ml) used, maximum inhibitory zone was observed at 300 mg/ml followed by 200 mg/ml and 100 mg/ml. No zone of inhibition was observed in the negative DMSO control. Methanol extract showed more inhibitory effect when compared to other solvents. Among the three plants, maximum inhibition activity was exhibited by Phyllanthus amarus followed by Azadirachta indica and Ocimum sanctum.